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Addgene

pD21-hgAPOAI
(Plasmid #124962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD21
  • Backbone manufacturer
    BCM
  • Backbone size w/o insert (bp) 24000
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human APOAI gene
  • Species
    H. sapiens (human)
  • Entrez Gene
    LPA (a.k.a. AK38, APOA, LP)
  • Promoter human APOAI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note plasmid was difficult to sequence due to its size. Addgene NGS results contain several ambiguous bases. We advise running additional diagnostic tests before use.

The backbone was developed at BCM. The relevant reference is Proc. Natl. Acad Sci. USA 98: 13282-13287.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD21-hgAPOAI was a gift from Kazuhiro Oka (Addgene plasmid # 124962 ; http://n2t.net/addgene:124962 ; RRID:Addgene_124962)

  • For your References section:

    Long-term stable expression of human apolipoprotein A-I mediated by helper-dependent adenovirus gene transfer inhibits atherosclerosis progression and remodels atherosclerotic plaques in a mouse model of familial hypercholesterolemia. Belalcazar LM, Merched A, Carr B, Oka K, Chen KH, Pastore L, Beaudet A, Chan L. Circulation. 2003 Jun 3;107(21):2726-32. doi: 10.1161/01.CIR.0000066913.69844.B2. Epub 2003 May 12. 10.1161/01.CIR.0000066913.69844.B2 PubMed 12742997
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