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Addgene

Flag-TRIM28
(Plasmid #124960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMX-(Flag)x2
  • Backbone manufacturer
    Barton Lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hTRIM28
  • Species
    H. sapiens (human)
  • Entrez Gene
    TRIM28 (a.k.a. KAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site - (unknown if destroyed)
  • 3′ cloning site - (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-TRIM28 was a gift from Michelle Barton (Addgene plasmid # 124960 ; http://n2t.net/addgene:124960 ; RRID:Addgene_124960)

  • For your References section:

    TRIM28 interacts with EZH2 and SWI/SNF to activate genes that promote mammosphere formation. Li J, Xi Y, Li W, McCarthy RL, Stratton SA, Zou W, Li W, Dent SY, Jain AK, Barton MC. Oncogene. 2017 May 25;36(21):2991-3001. doi: 10.1038/onc.2016.453. Epub 2017 Jan 9. 10.1038/onc.2016.453 PubMed 28068325
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