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Addgene

p270 pCMV-CRE-M-AR(LNCaP)
(Plasmid #12496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Cre-M (BglII)
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRE-M AR
  • Alt name
    androgen receptor
  • Species
    H. sapiens (human)
  • Mutation
    Thr 868 Ala in AR-LBD
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p270 pCMV-CRE-M-AR(LNCaP) was a gift from Jeffrey Green (Addgene plasmid # 12496 ; http://n2t.net/addgene:12496 ; RRID:Addgene_12496)

  • For your References section:

    Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Kaczmarczyk SJ, Green JE. Nucleic Acids Res. 2003 Aug 1. 31(15):e86. 10.1093/nar/gng087 PubMed 12888538
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