HA-TRIM28deltaBCC
(Plasmid #124954)

• Purpose: Mammalian expression of Human TRIM28 with deleted Bbox and CC domains
• Depositing Lab: Michelle Barton
• Publication: Li et al Oncogene. 2017 May 25;36(21):2991-3001. doi: 10.1038/onc.2016.453. Epub 2017 Jan 9.

Backbone

• Vector backbone: pCS2
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hTRIM28
• Species: H. sapiens (human)
• Mutation: Deletion of Bbox and CC domains
• Entrez Gene: TRIM28 (a.k.a. KAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert contains P590S mutation (P350S in CC domain deleted peptide)
Expected to have no impact on plasmid function.