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p264 pSP72 hAR-LBD(LNCaP)
(Plasmid #12495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP72
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AR LBD
  • Alt name
    androgen receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    903
  • Mutation
    Thr 878 Ala in AR-LBD
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid p258 was
digested with BglI and BsgI and the 1.8 kb fragment was
isolated. A second aliquot of p258 was digested with BglI and
DraIII and the 1.3 kb fragment was isolated. Both of these
fragments were ligated to a double-stranded oligonucleotide
resulting in p264.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p264 pSP72 hAR-LBD(LNCaP) was a gift from Jeffrey Green (Addgene plasmid # 12495 ; http://n2t.net/addgene:12495 ; RRID:Addgene_12495)

  • For your References section:

    Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Kaczmarczyk SJ, Green JE. Nucleic Acids Res. 2003 Aug 1. 31(15):e86. 10.1093/nar/gng087 PubMed 12888538
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