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Addgene

pET_MBP-RGG-RGG
(Plasmid #124946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBP-RGG-RGG (using LAF-1 RGG domain)
  • Species
    C. elegans (nematode)
  • Entrez Gene
    laf-1 (a.k.a. CELE_Y71H2AM.19)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • MBP (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET_MBP-RGG-RGG was a gift from Matthew Good & Daniel Hammer & Benjamin Schuster (Addgene plasmid # 124946 ; http://n2t.net/addgene:124946 ; RRID:Addgene_124946)

  • For your References section:

    Controllable protein phase separation and modular recruitment to form responsive membraneless organelles. Schuster BS, Reed EH, Parthasarathy R, Jahnke CN, Caldwell RM, Bermudez JG, Ramage H, Good MC, Hammer DA. Nat Commun. 2018 Jul 30;9(1):2985. doi: 10.1038/s41467-018-05403-1. 10.1038/s41467-018-05403-1 PubMed 30061688
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