pcDNA_RGG-RFP-RGG
(Plasmid
#124934)
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PurposeExpression of RGG-RFP-RGG
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRGG-RFP-RGG (using LAF-1 RGG domain)
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SpeciesC. elegans (nematode)
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Entrez Genelaf-1 (a.k.a. CELE_Y71H2AM.19)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA_RGG-RFP-RGG was a gift from Matthew Good & Daniel Hammer & Benjamin Schuster (Addgene plasmid # 124934 ; http://n2t.net/addgene:124934 ; RRID:Addgene_124934) -
For your References section:
Controllable protein phase separation and modular recruitment to form responsive membraneless organelles. Schuster BS, Reed EH, Parthasarathy R, Jahnke CN, Caldwell RM, Bermudez JG, Ramage H, Good MC, Hammer DA. Nat Commun. 2018 Jul 30;9(1):2985. doi: 10.1038/s41467-018-05403-1. 10.1038/s41467-018-05403-1 PubMed 30061688