p259 pCMV-CRE-M (BglII)
(Plasmid #12493)

• Depositing Lab: Jeffrey Green
• Publication: Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e86.

Backbone

• Vector backbone: pBS185
• Backbone size w/o insert (bp): 6100
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRE-M
• Alt name: modified Cre

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (unknown if destroyed)
• 3′ cloning site: See map (unknown if destroyed)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 3' coding sequence
for CRE-M contained in plasmid
p218 was PCR amplified.
The 812 bp amplification
product was digested with BamHI and MluI, gel purified and
ligated together with both the BamHI and MscI fragment from
p218 containing the 5' end of CRE-M and the MscI-MluI
fragment from pBS185 containing a polyA sequence.