p258 pSP72 hAR-LBD
(Plasmid #12492)

• Depositing Lab: Jeffrey Green
• Publication: Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e86.

Backbone

• Vector backbone: pSP72
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AR LBD
• Alt name: androgen receptor
• Species: H. sapiens (human)
• Insert Size (bp): 903
• Mutation: AR ligand binding domain
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry