pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2
(Plasmid
#124903)
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PurposeLentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti _Blast_DEST
- Backbone size w/o insert (bp) 7206
- Total vector size (bp) 11048
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep65 promoter- p65 CDS-mRuby2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3842
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Tag
/ Fusion Protein
- mRuby (C terminal on insert)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2 was a gift from Markus Covert (Addgene plasmid # 124903 ; http://n2t.net/addgene:124903 ; RRID:Addgene_124903) -
For your References section:
Escalating Threat Levels of Bacterial Infection Can Be Discriminated by Distinct MAPK and NF-kappaB Signaling Dynamics in Single Host Cells. Lane K, Andres-Terre M, Kudo T, Monack DM, Covert MW. Cell Syst. 2019 Mar 27;8(3):183-196.e4. doi: 10.1016/j.cels.2019.02.008. Epub 2019 Mar 20. 10.1016/j.cels.2019.02.008 PubMed 30904375