pBabe-Flag-Daxx-Puro
(Plasmid
#124898)
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PurposeExpress Flag-Daxx in mamalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-puro
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDeath domain-associated protein 6
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Insert Size (bp)2358
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Entrez GeneDAXX (a.k.a. BING2, DAP6, EAP1, SMIM40)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBabe 5'
- 3′ sequencing primer pBabe 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-Flag-Daxx-Puro was a gift from Xiaolu Yang (Addgene plasmid # 124898 ; http://n2t.net/addgene:124898 ; RRID:Addgene_124898) -
For your References section:
Phosphorylation of Daxx by ATM contributes to DNA damage-induced p53 activation. Tang J, Agrawal T, Cheng Q, Qu L, Brewer MD, Chen J, Yang X. PLoS One. 2013;8(2):e55813. doi: 10.1371/journal.pone.0055813. Epub 2013 Feb 6. 10.1371/journal.pone.0055813 PubMed 23405218