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Addgene

pMan operon
(Plasmid #124888)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124888 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pND50
  • Total vector size (bp) 9018
  • Vector type
    Bacterial Expression
  • Selectable markers
    Chloramphenicol

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    manX1, manX2, manY, manZ, manO, EF0025
  • Alt name
    manX1, manX2, manY, manZ, manO, EF0025
  • Alt name
    Man operon
  • Species
    E. faecalis
  • Insert Size (bp)
    5266
  • GenBank ID
    NP_813830.1, NP_813831.1, NP_813832.1, NP_813833.1, NP_813834.1, and NP_813835.1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCCGGGGATCCTCTGCTCTTAATATGGATATTCCAGCAGAAGAA
  • 3′ sequencing primer CTGCAGGTCGACTCTAAAATTTTAGTTCCCGTAGCGATTATCGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMan operon was a gift from Kazuhisa Sekimizu (Addgene plasmid # 124888 ; http://n2t.net/addgene:124888 ; RRID:Addgene_124888)

  • For your References section:

    Enterococcus faecalis YM0831 suppresses sucrose-induced hyperglycemia in a silkworm model and in humans. Matsumoto Y, Ishii M, Hasegawa S, Sekimizu K. Commun Biol. 2019 May 2;2:157. doi: 10.1038/s42003-019-0407-5. eCollection 2019. 10.1038/s42003-019-0407-5 PubMed 31069266
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