pET15bHis6-pG-MNase
(Plasmid
#124886)
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PurposeTo transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15bHisTnp (addgene# 79807)
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Modifications to backbonecut pET15bHisTnp with NdeI and BamHI. do Gibson NEBuilder with protein G's gBLOCK ordered from IDTDNA and PCR for MNase.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameprotein G-MNase
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Insert Size (bp)1143
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15bHis6-pG-MNase was a gift from Keji Zhao (Addgene plasmid # 124886 ; http://n2t.net/addgene:124886 ; RRID:Addgene_124886)
Map uploaded by the depositor.
