pET15b-pA-Mnase
(Plasmid
#124883)
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PurposeTo transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameprotein A-MNase
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Insert Size (bp)1461
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-pA-Mnase was a gift from Keji Zhao (Addgene plasmid # 124883 ; http://n2t.net/addgene:124883 ; RRID:Addgene_124883) -
For your References section:
Profiling single-cell histone modifications using indexing chromatin immunocleavage sequencing. Ku WL, Pan L, Cao Y, Gao W, Zhao K. Genome Res. 2021 Apr 14. pii: gr.260893.120. doi: 10.1101/gr.260893.120. 10.1101/gr.260893.120 PubMed 33853847
Map uploaded by the depositor.
