pCAD96_hIRG1_4-461_pvp008
(Plasmid
#124874)
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PurposeExpresses hCAD Asn152Ser mutant in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124874 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCOLADuet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3519
- Total vector size (bp) 4893
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Modifications to backboneN-terminal StrepTag and TEV site
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecis-aconitate decarboxylase
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Alt nameIrg1
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Alt nameCAD
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SpeciesH. sapiens (human)
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Insert Size (bp)1374
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Mutationmutated Asn152 to Ser, deleted amino acids 1-3 and 462-end.
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GenBank IDNM_001258406
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Entrez GeneACOD1 (a.k.a. CAD, IRG1)
- Promoter T7
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Tag
/ Fusion Protein
- StrepTag and TEV site (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TACGACTCACTATAGGGGAATTGTG
- 3′ sequencing primer TGCTAGTTATTGCTCAGCGGTGGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAD96_hIRG1_4-461_pvp008 was a gift from Konrad Buessow (Addgene plasmid # 124874 ; http://n2t.net/addgene:124874 ; RRID:Addgene_124874) -
For your References section:
Crystal structure of cis-aconitate decarboxylase reveals the impact of naturally occurring human mutations on itaconate synthesis. Chen F, Lukat P, Iqbal AA, Saile K, Kaever V, van den Heuvel J, Blankenfeldt W, Bussow K, Pessler F. Proc Natl Acad Sci U S A. 2019 Sep 23. pii: 1908770116. doi: 10.1073/pnas.1908770116. 10.1073/pnas.1908770116 PubMed 31548418