SPOON/ pcDNA3
(Plasmid
#124840)
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Purposemammalian expression of SPOON, a spontaneously blinking green fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPOON
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SpeciesSynthetic
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Insert Size (bp)700
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GenBank IDLC320161
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Tag
/ Fusion Protein
- No tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SPOON/ pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 124840 ; http://n2t.net/addgene:124840 ; RRID:Addgene_124840) -
For your References section:
Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T, Nagai T. ACS Chem Biol. 2018 Aug 17;13(8):1938-1943. doi: 10.1021/acschembio.8b00200. Epub 2018 Jul 10. 10.1021/acschembio.8b00200 PubMed 29963852
Map uploaded by the depositor.
