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Addgene

MARIO/ pcDNA3
(Plasmid #124838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5421
  • Total vector size (bp) 7446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MARIO
  • Species
    Synthetic
  • Insert Size (bp)
    2025
  • GenBank ID
    LC316970
  • Promoter CMV
  • Tag / Fusion Protein
    • No tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MARIO/ pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 124838 ; http://n2t.net/addgene:124838 ; RRID:Addgene_124838)

  • For your References section:

    A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T. Curr Biol. 2018 Feb 5;28(3):444-451.e6. doi: 10.1016/j.cub.2017.12.035. Epub 2018 Jan 18. 10.1016/j.cub.2017.12.035 PubMed 29358072
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