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Purposemammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGamillus
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SpeciesSynthetic
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Insert Size (bp)700
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GenBank IDLC309249
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Tag
/ Fusion Protein
- No tag
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gamillus/ pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 124837 ; http://n2t.net/addgene:124837 ; RRID:Addgene_124837) -
For your References section:
Acid-Tolerant Monomeric GFP from Olindias formosa. Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T. Cell Chem Biol. 2018 Mar 15;25(3):330-338.e7. doi: 10.1016/j.chembiol.2017.12.005. Epub 2017 Dec 28. 10.1016/j.chembiol.2017.12.005 PubMed 29290624