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pHHM.X513-iNicSnFR2-(CC90)
(Plasmid #124836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHHM.X513
  • Backbone manufacturer
    Janelia
  • Backbone size w/o insert (bp) 2827
  • Total vector size (bp) 4444
  • Modifications to backbone
    NA
  • Vector type
    Bacterial Expression
  • Selectable markers
    NA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for bacterial expression and grow at 30 degrees C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iNicSnFR2-(CC90)
  • Alt name
    iNicSnFR2-(CC90)
  • Species
    Bacteria
  • Insert Size (bp)
    1617
  • Mutation
    NA
  • Promoter T7
  • Tags / Fusion Proteins
    • His Tag (N terminal on insert)
    • HA tags (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site HindiIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector was received from Janelia HHMI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHHM.X513-iNicSnFR2-(CC90) was a gift from Henry Lester (Addgene plasmid # 124836 ; http://n2t.net/addgene:124836 ; RRID:Addgene_124836)

  • For your References section:

    Determining the pharmacokinetics of nicotinic drugs in the endoplasmic reticulum using biosensors. Shivange AV, Borden PM, Muthusamy AK, Nichols AL, Bera K, Bao H, Bishara I, Jeon J, Mulcahy MJ, Cohen B, O'Riordan SL, Kim C, Dougherty DA, Chapman ER, Marvin JS, Looger LL, Lester HA. J Gen Physiol. 2019 Feb 4. pii: jgp.201812201. doi: 10.1085/jgp.201812201. 10.1085/jgp.201812201 PubMed 30718376
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