nLuc-GLP-1R-His-FLAG
(Plasmid #124831)

• Purpose: Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells
• Depositing Lab: Sam Gellman
• Publication: Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. doi: 10.1038/s41589-021-00945-w. Epub 2021 Dec 22.

Backbone

• Vector backbone: pSectag2
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 7200
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GLP1R
• Alt name: Glucagon-like peptide-1 receptor
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1959
• GenBank ID: AAI12127.1
• Entrez Gene: GLP1R (a.k.a. GLP-1, GLP-1-R, GLP-1R)
• Promoter: CMV
• Tags / Fusion Proteins:
  • NanoLuc (N terminal on insert)
  • His6 (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DNA encoding Nanoluc was kindly provided by Promega. GLP-1R cloned in a modifed pSectag2 was kindly provided by Pfizer (Nolte, et al. “A Potentiator of Orthosteric Ligand Activity at GLP-1R Acts via Covalent Modification.” Nat. Chem. Biol. 10, no. 8 (2014): 629–31.) .

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.02.24.432589v1 for bioRxiv preprint.