Mito-PyronicSF/pCMV-myc-mito
(Plasmid #124813)

• Purpose: Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.
• Depositing Lab: Luis Felipe Barros
• Publication: Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10.7554/eLife.53917.

Backbone

• Vector backbone: pCMV-myc-mito
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 4973
• Total vector size (bp): 6473
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PyronicSF
• Alt name: Pyruvate Optical Nano-Indicator from CECs Single-Fluorophore
• Species: Synthetic
• Insert Size (bp): 1500
• Promoter: CMV
• Tag / Fusion Protein:
  • Mitochondrial Sequence Signal (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/611806v1 for BioRxiv preprint