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Addgene

Top7 S2-S4
(Plasmid #12478)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12478 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pet29 b(+)
  • Backbone size w/o insert (bp) 5371
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Top7 S2-S4
  • Insert Size (bp)
    249
  • Mutation
    S2-S4
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Top7 S2-S4 was a gift from David Baker (Addgene plasmid # 12478 ; http://n2t.net/addgene:12478 ; RRID:Addgene_12478)