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Purpose(Empty Backbone) 3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLenti-Cas9-gRNA-GFP (Addgene #124770)
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Modifications to backboneTagBFP2 swapped in for GFP in Addgene #124770
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersTagBFP2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-Cas9-gRNA-TagBFP2 was a gift from Jason Sheltzer (Addgene plasmid # 124774 ; http://n2t.net/addgene:124774 ; RRID:Addgene_124774) -
For your References section:
Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9. Giuliano CJ, Lin A, Girish V, Sheltzer JM. Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100. 10.1002/cpmb.100 PubMed 31503414
