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Purpose(Empty Backbone) Lentiviral plasmid to co-express a guide RNA and TagBFP2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLRG2.1 (Addgene #108098)
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Modifications to backboneThe LRG2.1 plasmid in which GFP has been replaced with TagBFP2.
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersTagBFP2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LRG2.1-TagBFP2 was a gift from Jason Sheltzer (Addgene plasmid # 124773 ; http://n2t.net/addgene:124773 ; RRID:Addgene_124773) -
For your References section:
Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9. Giuliano CJ, Lin A, Girish V, Sheltzer JM. Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100. 10.1002/cpmb.100 PubMed 31503414