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Addgene

Lenti-Cas9-gRNA-GFP
(Plasmid #124770)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124770 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    LRG2.1 (Addgene #108098)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Provided by Chris Vakoc
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-Cas9-gRNA-GFP was a gift from Jason Sheltzer (Addgene plasmid # 124770 ; http://n2t.net/addgene:124770 ; RRID:Addgene_124770)
  • For your References section:

    Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9. Giuliano CJ, Lin A, Girish V, Sheltzer JM. Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100. 10.1002/cpmb.100 PubMed 31503414