Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
(Plasmid
#12474)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet29 b(+)
- Backbone size w/o insert (bp) 5371
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTop7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
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Insert Size (bp)324
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Mutationb19 only. N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L.
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L was a gift from David Baker (Addgene plasmid # 12474 ; http://n2t.net/addgene:12474 ; RRID:Addgene_12474)