pKD251
(Plasmid #124714)

• Purpose: Expression of torS under Ptac and torT under PD59_luxR
• Depositing Lab: Jeffrey Tabor
• Publication: Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. doi: 10.1038/s41589-019-0286-6. Epub 2019 May 20.

Backbone

• Vector backbone: pACYCDuet-1
• Total vector size (bp): 8865
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: torS
• Insert Size (bp): 2742
• Entrez Gene: torS (a.k.a. b0993, ECK0984, yccI)

Gene/Insert 2

• Gene/Insert name: torT
• Insert Size (bp): 1026
• Entrez Gene: torS (a.k.a. b0993, ECK0984, yccI)

Gene/Insert 3

• Gene/Insert name: lacI
• Insert Size (bp): 1080
• Entrez Gene: lacI (a.k.a. b0345, ECK0342)

Gene/Insert 4

• Gene/Insert name: luxR
• Species: Aliivibrio fischeri
• Insert Size (bp): 750
• GenBank ID: AF170104

Resource Information

• Supplemental Documents:
  • pkd251.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry