pSR348
(Plasmid #124713)

• Purpose: Expression of narX under Ptac
• Depositing Lab: Jeffrey Tabor
• Publication: Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. doi: 10.1038/s41589-019-0286-6. Epub 2019 May 20.

Backbone

• Vector backbone: pACYCDuet-1
• Total vector size (bp): 5088
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: narX
• Insert Size (bp): 1794
• Entrez Gene: narX (a.k.a. b1222, ECK1216, frd1, frdR, narR)

Gene/Insert 2

• Gene/Insert name: lacI
• Insert Size (bp): 1080
• Mutation: S193* - LacI is truncated and likely deactivated. Please see depositor comments below.
• Entrez Gene: lacI (a.k.a. b0345, ECK0342)

Resource Information

• Supplemental Documents:
  • psr348.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

LacI is truncated and likely deactivated in this plasmid. This causes NarX, which is transcribed via the LacI repressible promoter Ptac, to be constitutively expressed.