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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS
(Plasmid #124705)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124705 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MCP_ADAR2 DD (E488Q)_NLS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1620
  • Mutation
    E488Q hyperactive mutant
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer TCCTTCCGTGTTTCAGTTAGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also expresses MS2_adRNA targeting the RAB7A transcript

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS was a gift from Prashant Mali (Addgene plasmid # 124705 ; http://n2t.net/addgene:124705 ; RRID:Addgene_124705)

  • For your References section:

    In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Katrekar D, Chen G, Meluzzi D, Ganesh A, Worlikar A, Shih YR, Varghese S, Mali P. Nat Methods. 2019 Mar;16(3):239-242. doi: 10.1038/s41592-019-0323-0. Epub 2019 Feb 8. 10.1038/s41592-019-0323-0 PubMed 30737497
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