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Addgene

pLenti-PGK-Venus-Akaluc (neo)
(Plasmid #124701)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124701 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti PGK Neo DEST (Addgene #19067)
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman
  • Backbone size w/o insert (bp) 9780
  • Total vector size (bp) 10575
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Venus-Akaluciferase
  • Species
    Synthetic; Aequorea victoria (Venus) and Photinus pyralis (Akaluciferase)
  • Insert Size (bp)
    2400
  • Promoter hPGK

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    the Venus-Akaluc insert was obtained from a plasmid that we received with an MTA from the RIKEN BRC (bioresource center), Tsukuba, Japan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PGK-Venus-Akaluc (neo) was a gift from Roland Friedel (Addgene plasmid # 124701 ; http://n2t.net/addgene:124701 ; RRID:Addgene_124701)

  • For your References section:

    Akaluc bioluminescence offers superior sensitivity to track in vivo glioma expansion. Bozec D, Sattiraju A, Bouras A, Jesu Raj JG, Rivera D, Huang Y, Junqueira Alves C, Tejero R, Tsankova NM, Zou H, Hadjipanayis C, Friedel RH. Neurooncol Adv. 2020 Oct 10;2(1):vdaa134. doi: 10.1093/noajnl/vdaa134. eCollection 2020 Jan-Dec. 10.1093/noajnl/vdaa134 PubMed 33241215
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