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Addgene

5538 pUG2-SSA1
(Plasmid #1247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUG2
  • Backbone size w/o insert (bp) 8700
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ssa1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2900
  • Entrez Gene
    SSA1 (a.k.a. YAL005C, YG100)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site bamh1 (not destroyed)
  • 3′ cloning site sac1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    John Glover Elizabeth Craig

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5538 pUG2-SSA1 was a gift from Susan Lindquist (Addgene plasmid # 1247 ; http://n2t.net/addgene:1247 ; RRID:Addgene_1247)

  • For your References section:

    Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins. Glover JR, Lindquist S. Cell 1998 Jul 10;94(1):73-82. 10.1016/S0092-8674(00)81223-4 PubMed 9674429
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