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Addgene

OVA-miSFIT-2xPerfect
(Plasmid #124673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE
  • Backbone manufacturer
    Addgene: 85866
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cyto-Ovalbumin
  • Species
    G. gallus (chicken)
  • Mutation
    Truncated OVA for cytoplasmic expression
  • Promoter minimal CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bi-directional expression vector driving OVA-T2A-EGFP under control of a miR-17 MRE with NGFR as an internal control

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OVA-miSFIT-2xPerfect was a gift from Tudor Fulga (Addgene plasmid # 124673 ; http://n2t.net/addgene:124673 ; RRID:Addgene_124673)

  • For your References section:

    Precise tuning of gene expression levels in mammalian cells. Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, Milne TA, Cerundolo V, Fulga TA. Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y. 10.1038/s41467-019-08777-y PubMed 30778069
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