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PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124663 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST14
- Backbone size w/o insert (bp) 3648
- Total vector size (bp) 4242
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpyCatcher002-oTet
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SpeciesSynthetic
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Insert Size (bp)594
- Promoter T7
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Tags
/ Fusion Proteins
- His6 (N terminal on insert)
- C-tag (C terminal on insert)
- TEV cleavage site (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SpyCatcher002-oTet was a gift from Mark Howarth (Addgene plasmid # 124663 ; http://n2t.net/addgene:124663 ; RRID:Addgene_124663) -
For your References section:
Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox. Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, Howarth M. Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w. 10.1038/s41467-019-09678-w PubMed 30988307
