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Addgene

Src-Y530F
(Plasmid #124659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF6
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cSrc
  • Alt name
    c-Src with Y530F
  • Species
    H. sapiens (human)
  • Mutation
    Y530F
  • Entrez Gene
    SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Src-Y530F was a gift from Cheng-han Yu (Addgene plasmid # 124659 ; http://n2t.net/addgene:124659 ; RRID:Addgene_124659)

  • For your References section:

    Tail domains of myosin-1e regulate phosphatidylinositol signaling and F-actin polymerization at the ventral layer of podosomes. Zhang Y, Cao F, Zhou Y, Feng Z, Sit B, Krendel M, Yu CH. Mol Biol Cell. 2019 Mar 1;30(5):622-635. doi: 10.1091/mbc.E18-06-0398. Epub 2019 Jan 2. 10.1091/mbc.E18-06-0398 PubMed 30601698
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