EBFP2-UtrCH
(Plasmid #124656)

• Purpose: F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).
• Depositing Lab: Cheng-han Yu
• Publication: Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635. doi: 10.1091/mbc.E18-06-0398. Epub 2019 Jan 2.

Backbone

• Vector backbone: pCS2+
• Modifications to backbone: Subcloned from Addgene #26737. Original GFP is replaced by EBFP2.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Utrophin
• Alt name: Calponin homology domain of utrophin (aa# 1-261)
• Species: H. sapiens (human)
• Mutation: see depositor comments below
• GenBank ID: NM_007124.2
• Entrez Gene: UTRN (a.k.a. DMDL, DRP, DRP1)
• Tag / Fusion Protein:
  • EBFP2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation Q236R in utrophin was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.