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Addgene

EBFP2-UtrCH
(Plasmid #124656)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Modifications to backbone
    Subcloned from Addgene #26737. Original GFP is replaced by EBFP2.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Utrophin
  • Alt name
    Calponin homology domain of utrophin (aa# 1-261)
  • Species
    H. sapiens (human)
  • Mutation
    see depositor comments below
  • GenBank ID
    NM_007124.2
  • Entrez Gene
    UTRN (a.k.a. DMDL, DRP, DRP1)
  • Tag / Fusion Protein
    • EBFP2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation Q236R in utrophin was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EBFP2-UtrCH was a gift from Cheng-han Yu (Addgene plasmid # 124656 ; http://n2t.net/addgene:124656 ; RRID:Addgene_124656)
  • For your References section:

    Tail domains of myosin-1e regulate phosphatidylinositol signaling and F-actin polymerization at the ventral layer of podosomes. Zhang Y, Cao F, Zhou Y, Feng Z, Sit B, Krendel M, Yu CH. Mol Biol Cell. 2019 Mar 1;30(5):622-635. doi: 10.1091/mbc.E18-06-0398. Epub 2019 Jan 2. 10.1091/mbc.E18-06-0398 PubMed 30601698