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Addgene

Cytosolic Fyn FRET Biosensor
(Plasmid #124654)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124654 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone manufacturer
    Michiyuki Matsuda lab at Tokyo University / Addgene
  • Backbone size w/o insert (bp) 4739
  • Total vector size (bp) 6521
  • Modifications to backbone
    Sal I in the backbone was mutated.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
  • Alt name
    Cytosolic Fyn Biosensor
  • Insert Size (bp)
    1782

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cytosolic Fyn FRET Biosensor was a gift from Yingxiao Wang (Addgene plasmid # 124654 ; http://n2t.net/addgene:124654 ; RRID:Addgene_124654)

  • For your References section:

    Sensitive FRET Biosensor Reveals Fyn Kinase Regulation by Submembrane Localization. Ouyang M, Wan R, Qin Q, Peng Q, Wang P, Wu J, Allen M, Shi Y, Laub S, Deng L, Lu S, Wang Y. ACS Sens. 2019 Jan 25;4(1):76-86. doi: 10.1021/acssensors.8b00896. Epub 2019 Jan 9. 10.1021/acssensors.8b00896 PubMed 30588803
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