pcDNA-PU.1-ETS
(Plasmid #124652)

• Purpose: Chimeric ETS domain
• Depositing Lab: Gregory Poon
• Publication: Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 10.1093/nar/gky894.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 5800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use Hek293 cells for expression in mammalian cells.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PU.1 ETS domain (residues 167-272)
• Species: H. sapiens (human)
• Insert Size (bp): 318
• Entrez Gene: SPI1 (a.k.a. AGM10, OF, PU.1, SFPI1, SPI-1, SPI-A)
• Entrez Gene: ETS1 (a.k.a. ETS-1, EWSR2, c-ets-1, p54)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry