MS2_adRNA-MCP_ADAR1 DD_NLS
(Plasmid
#124625)
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PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124625 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backboneUnknown
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMCP_ADAR1 DD_NLS
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SpeciesH. sapiens (human)
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Insert Size (bp)1752
- Promoter CMV
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Tag
/ Fusion Protein
- NLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer TCCTTCCGTGTTTCAGTTAGCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also expresses MS2_adRNA targeting the RAB7A transcript
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MS2_adRNA-MCP_ADAR1 DD_NLS was a gift from Prashant Mali (Addgene plasmid # 124625 ; http://n2t.net/addgene:124625 ; RRID:Addgene_124625) -
For your References section:
In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Katrekar D, Chen G, Meluzzi D, Ganesh A, Worlikar A, Shih YR, Varghese S, Mali P. Nat Methods. 2019 Mar;16(3):239-242. doi: 10.1038/s41592-019-0323-0. Epub 2019 Feb 8. 10.1038/s41592-019-0323-0 PubMed 30737497