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Purposeproximity biotinylation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
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Selectable markersV5
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAPEX2-NLS
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
APEX2-NLS was a gift from Alice Ting (Addgene plasmid # 124617 ; http://n2t.net/addgene:124617 ; RRID:Addgene_124617) -
For your References section:
Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking. Kaewsapsak P, Shechner DM, Mallard W, Rinn JL, Ting AY. Elife. 2017 Dec 14;6. doi: 10.7554/eLife.29224. 10.7554/eLife.29224 PubMed 29239719
