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Addgene

pcDNA-PU.1-ETS N236Y
(Plasmid #124614)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use Hek293 cells for expression in mammalian cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PU.1 ETS domain (residues 167-272)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    318
  • Mutation
    Residues 236 altered from asparagine to a Tyrosine
  • Entrez Gene
    SPI1 (a.k.a. AGM10, OF, PU.1, SFPI1, SPI-1, SPI-A)
  • Entrez Gene
    ETS1 (a.k.a. ETS-1, EWSR2, c-ets-1, p54)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-PU.1-ETS N236Y was a gift from Gregory Poon (Addgene plasmid # 124614 ; http://n2t.net/addgene:124614 ; RRID:Addgene_124614)

  • For your References section:

    Mapping interfacial hydration in ETS-family transcription factor complexes with DNA: a chimeric approach. Albrecht AV, Kim HM, Poon GMK. Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 10.1093/nar/gky894. 10.1093/nar/gky894 PubMed 30295801
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