pJPL3[flp-6p::cmk-1::GFP]
(Plasmid
#124613)
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PurposeUsed to confirm the expression of the trans-spliced GFP reporter in the expected ASE neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST R4R3
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name[flp-6p::cmk-1::GFP]
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SpeciesC. elegans (nematode)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer ggggacaactttgtatagaaaagttgTCGCTTGACTTCTGATG
- 3′ sequencing primer ggggactgcttttttgtacaaacttgTTCTGGAATAATCATATTG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe original plasmid was provided by; Dr. Dominique Glauser Department of Biology University of Fribourg Chemin du Musee 10 1700 Fribourg Switzerland Plasmid generated by: Gabriella Saro.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJPL3[flp-6p::cmk-1::GFP] was a gift from Anne Brunet (Addgene plasmid # 124613 ; http://n2t.net/addgene:124613 ; RRID:Addgene_124613) -
For your References section:
Loss of CaMKI Function Disrupts Salt Aversive Learning in C. elegans. Lim JP, Fehlauer H, Das A, Saro G, Glauser DA, Brunet A, Goodman MB. J Neurosci. 2018 Jul 4;38(27):6114-6129. doi: 10.1523/JNEUROSCI.1611-17.2018. Epub 2018 Jun 6. 10.1523/JNEUROSCI.1611-17.2018 PubMed 29875264