pSCM001
(Plasmid #124605)

• Purpose: Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
• Depositing Lab: David Summers
• Publication: Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1038/s41598-019-40560-3.

Backbone

• Vector backbone: pBAD TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4127
• Total vector size (bp): 5433
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-pHluorin
• Alt name: mCherry-Super Ecliptic pHluorin
• Species: E. coli
• Insert Size (bp): 1440
• GenBank ID:
• Promoter: pDAB
• Tag / Fusion Protein:
  • mCherry-(Gly-Gly-Ser)x2-pHluorin translational fusion protein

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACATGGCATGGATGAACTATACAAATGAAAACGGTCTCCAGCTTGGCT
• 3′ sequencing primer: TCGCCATGTTGTCTTCTTCGCCTTTTGAAACCATGGTATGTATATCTCCTTCTTAAAGTTAAACA

Resource Information

• Supplemental Documents:
  • mcherry-phluorin.gb
  • Expression of pSCM001 in E. coli
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry