pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40
(Plasmid
#124604)
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PurposeCre-dependent soma-localized C1V1(t/t) for optogenetic stimulation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 7301
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSoma-localized C1V1(t/t)
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SpeciesM. musculus (mouse)
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Insert Size (bp)3367
- Promoter Ef1a
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Tag
/ Fusion Protein
- mRuby2 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ---
- 3′ sequencing primer --- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40 was a gift from Christopher Harvey (Addgene plasmid # 124604 ; http://n2t.net/addgene:124604 ; RRID:Addgene_124604) -
For your References section:
Single-neuron perturbations reveal feature-specific competition in V1. Chettih SN, Harvey CD. Nature. 2019 Mar 6. pii: 10.1038/s41586-019-0997-6. doi: 10.1038/s41586-019-0997-6. 10.1038/s41586-019-0997-6 PubMed 30842660