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Addgene

pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40
(Plasmid #124604)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124604 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 7301
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Soma-localized C1V1(t/t)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3367
  • Promoter Ef1a
  • Tag / Fusion Protein
    • mRuby2 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40 was a gift from Christopher Harvey (Addgene plasmid # 124604 ; http://n2t.net/addgene:124604 ; RRID:Addgene_124604)
  • For your References section:

    Single-neuron perturbations reveal feature-specific competition in V1. Chettih SN, Harvey CD. Nature. 2019 Mar 6. pii: 10.1038/s41586-019-0997-6. doi: 10.1038/s41586-019-0997-6. 10.1038/s41586-019-0997-6 PubMed 30842660