pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40
(Plasmid #124604)

• Purpose: Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation
• Depositing Lab: Christopher Harvey
• Publication: Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-019-0997-6. doi: 10.1038/s41586-019-0997-6.

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Backbone

• Vector backbone: pcDNA
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 7301
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Soma-localized C1V1(t/t)
• Species: M. musculus (mouse)
• Insert Size (bp): 3367
• Promoter: Ef1a
• Tag / Fusion Protein:
  • mRuby2 (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: ---
• 3′ sequencing primer: ---

Resource Information

• Supplemental Documents:
  • pAAV-EF1a-DIO-C1V1(t_t)-mRuby2-KV21TS-WPRE-SV40.gbk
• A portion of this plasmid was derived from a plasmid made by: Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry