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Addgene

5228 pG-N795
(Plasmid #1246)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1246 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pG1
  • Backbone size w/o insert (bp) 7400
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glucocorticoid receptor
  • Species
    n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site hind111 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    John Glover

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional Reference: Glover JR and Lindquist S. 1998. Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins.
Cell Jul 10;94(1):73-82.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5228 pG-N795 was a gift from Susan Lindquist (Addgene plasmid # 1246)

  • For your References section:

    Reduced levels of hsp90 compromise steroid receptor action in vivo. Picard D, Khursheed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto KR. Nature 1990 Nov 8;348(6297):166-8. 10.1038/348166a0 PubMed 2234079
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