CellTagB
(Plasmid #124592)

• Purpose: Expressed barcode for sample multiplexing in single-cell analysis
• Depositing Lab: Samantha Morris
• Publication: Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 10.1186/s13059-019-1699-y.

Backbone

• Vector backbone: pSMAL
• Backbone size w/o insert (bp): 8426
• Total vector size (bp): 8432
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CellTagB
• Species: Synthetic
• Insert Size (bp): 8
• Promoter: hCMV

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: SV40 PolyA

Resource Information

• Supplemental Documents:
  • CellTagB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014).

Please visit https://www.biorxiv.org/content/10.1101/335547v2 for bioRxiv preprint.