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Addgene

pRB895
(Plasmid #124586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFS2A
  • Backbone manufacturer
    PMID: 15474295
  • Backbone size (bp) 7509
  • Vector type
    Synthetic Biology ; Candida albicans expression
  • Selectable markers
    Nourseothricin
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The different orientation of the F1 ori does not alter the plasmid's function. Please refer to the the Addgene sequence for the most accurate result.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB895 was a gift from Richard Bennett (Addgene plasmid # 124586 ; http://n2t.net/addgene:124586 ; RRID:Addgene_124586)

  • For your References section:

    Monitoring Phenotypic Switching in Candida albicans and the Use of Next-Gen Fluorescence Reporters. Frazer C, Hernday AD, Bennett RJ. Curr Protoc Microbiol. 2019 Feb 12:e76. doi: 10.1002/cpmc.76. 10.1002/cpmc.76 PubMed 30747494
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