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Purpose(Empty Backbone) C-terminal fusions of C. albicans optimized mNeonGreen fluorescent protein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSFS2A
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Backbone manufacturerPMID: 15474295
- Backbone size (bp) 7509
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Vector typeSynthetic Biology ; Candida albicans expression
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Selectable markersNourseothricin
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Tag
/ Fusion Protein
- mNeonGreen (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRB895 was a gift from Richard Bennett (Addgene plasmid # 124586 ; http://n2t.net/addgene:124586 ; RRID:Addgene_124586) -
For your References section:
Monitoring Phenotypic Switching in Candida albicans and the Use of Next-Gen Fluorescence Reporters. Frazer C, Hernday AD, Bennett RJ. Curr Protoc Microbiol. 2019 Feb 12:e76. doi: 10.1002/cpmc.76. 10.1002/cpmc.76 PubMed 30747494