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pHO_pTEFmut7_CggR_R250A_ble
(Plasmid #124585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHO - plasmid
  • Modifications to backbone
    Insertion of the ble selection marker (resistance against phleomycin)
  • Vector type
    Synthetic Biology
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cggR_R250A
  • Species
    Synthetic
  • Mutation
    changed arginine 250 to alanine
  • Entrez Gene
    cggR (a.k.a. BSU_33950, BSU33950)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AAA ACT ATG GCT CAA GCA AGA AAC ACC CCA
  • 3′ sequencing primer TTT TGA TAC CGA GTT CGT TCT TTG TGG GGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHO_pTEFmut7_CggR_R250A_ble was a gift from Matthias Heinemann (Addgene plasmid # 124585 ; http://n2t.net/addgene:124585 ; RRID:Addgene_124585)

  • For your References section:

    Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor. Monteiro F, Hubmann G, Takhaveev V, Vedelaar SR, Norder J, Hekelaar J, Saldida J, Litsios A, Wijma HJ, Schmidt A, Heinemann M. Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071. 10.15252/msb.20199071 PubMed 31885198
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