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Addgene

pTEF6-7
(Plasmid #124583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124583 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p416
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418), URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer ATTAGGTACCCGAGCTCTCGAGAACCCTTAATATAACTT
  • 3′ sequencing primer ATATGGTACCAGCGTACGAGCGACCTCATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTEF6-7 was a gift from Matthias Heinemann (Addgene plasmid # 124583 ; http://n2t.net/addgene:124583 ; RRID:Addgene_124583)

  • For your References section:

    Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor. Monteiro F, Hubmann G, Takhaveev V, Vedelaar SR, Norder J, Hekelaar J, Saldida J, Litsios A, Wijma HJ, Schmidt A, Heinemann M. Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071. 10.15252/msb.20199071 PubMed 31885198
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