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pCR8_TaNAM-A1(1-217)
(Plasmid #124576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 2817
  • Total vector size (bp) 3471
  • Vector type
    TOPO Gateway Cloning Vector
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TaNAM-A1 (1-217)
  • Alt name
    TaGPC-A1 (1-217)
  • Species
    Triticum aestivum
  • Insert Size (bp)
    651
  • GenBank ID
    KM820886.1
  • Promoter None

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer CAGGAAACAGCTATGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR8_TaNAM-A1(1-217) was a gift from Cristobal Uauy (Addgene plasmid # 124576 ; http://n2t.net/addgene:124576 ; RRID:Addgene_124576)

  • For your References section:

    Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC domain are required for protein binding and when mutated lead to delayed peduncle and flag leaf senescence. Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C. BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 10.1186/s12870-019-2022-5. 10.1186/s12870-019-2022-5 PubMed 31533618
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