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pGWB12_TtNAM-B1
(Plasmid #124573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGWB12
  • Backbone size w/o insert (bp) 15622
  • Total vector size (bp) 16840
  • Modifications to backbone
    N/A
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TaNAM-B1
  • Alt name
    TaGPC-B1
  • Species
    Triticum turgidum ssp. dicoccoides
  • Insert Size (bp)
    1218
  • GenBank ID
    DQ869673.1
  • Promoter 35s
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ATGGGCAGCTCCGACTCA
  • 3′ sequencing primer TCAGGGATTCCAGTTCACGCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWB12_TtNAM-B1 was a gift from Cristobal Uauy (Addgene plasmid # 124573 ; http://n2t.net/addgene:124573 ; RRID:Addgene_124573)

  • For your References section:

    Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC domain are required for protein binding and when mutated lead to delayed peduncle and flag leaf senescence. Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C. BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 10.1186/s12870-019-2022-5. 10.1186/s12870-019-2022-5 PubMed 31533618
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